Advanced science: an anisotropic nucleic acid nanointerface for accurate capture of circulating tumor cells

In recent years non-invasive liquid biopsy has been used in disease monitoring drug resistance tracking. Among them circulating tumor cells provide a new scheme for cancer diagnosis monitoring medication guidance. Due to the low abundance of circulating tumor cells in the blood the effective capture of circulating tumor cells has become the focus of researchers. At present many methods for capturing circulating tumor cells have been developed including antigen-dependent cell capture label free cell separation. However these methods still face challenges in accurately capturing circulating tumor cells due to the inevitable heterogeneity of circulating tumor cells such as epithelial mesenchymal transition.

is inspired by the strong interaction between cells mediated by synergetic receptors in immune synapses. Professor Zhang Yuanqing his research team from school of pharmacy of Sun Yat sen University cooperated with associate researcher Li Xi of Shanghai Jiaotong University to design prepare anisotropic nano interfaces by utilizing the advantages of nucleic acid nanotechnology using nucleic acid tetrahedron as the frame to arrange different types of capture ligs And its characteristics are studied in detail. Firstly different from the isotropic nano interface the anisotropic nucleic acid nano interface can be effectively displayed on the surface of cell membrane expressing different degrees of epithelial cell adhesion molecules thus avoiding endocytosis. The effective anchoring of the nano interface on the cell membrane is due to the efficient cooperative recognition of different types of aptamers on the heterogeneous cell membrane. Combined with magnetic bead separation technology the nano interface can not only ensure the efficient capture of high expression of epithelial adhesion factor cells but also greatly improve the capture efficiency of low expression cells. It is worth noting that when enriching heterogeneous model cells the platform can avoid large changes in cell composition before after enrichment ensure the accuracy of circulating tumor cell capture. In addition the researchers also demonstrated the possibility of applying the nano interface to the detection of circulating tumor cells in clinical samples downstream detection. This bionic nano interface provides a new idea for improving the capture of circulating tumor cells is expected to further promote the development of nucleic acid nanotechnology cell membrane engineering. This work is supported by the National Natural Science Foundation of China the national key R & D program the natural science foundation of Guangdong Province.

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